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Production of 3.6 × 10 10 spores/mL, corresponding to a 5 folds Using the fed-batch strategy, it was possible to achieve a maximum spore Sulphate, using an exponential and a constant feeding profile, respectively. Glucose and calcium and the addition of a feeding solution of ammonium Intermediate fed-batch phase with the addition of a feeding solution containing Theĭeveloped strategy consisted of an initial and a final batch phase and an Nitrogen ratio at the end of the exponential growth phase studied. A fed-batchīioprocess was developed, being the effect on sporulation of the carbon to Vitamin composition, carbon, nitrogen and calcium concentrations. Medium composition was optimized with respect to In this study a new animal-free component,Ĭhemically defined medium, was tested for B. Potential source for the presence of adventious agents, therefore undesirableįor use in production scale. Media often include relatively expensive components of animal origin that are a Spores of Bacillus subtilis are being used as probiotics andĬompetitive exclusion agents for animal consumption.

Spore definition